未知题型

连续监测法测LD酶活性。取血清50ul，加LD-P法试剂1.0ml，混匀，置37℃中。第120s时在波长340nm处1cm光径测得吸光度为1.45，第150s时测得吸光度为1.40。已知NADH毫摩尔吸光系数为6.3L cm-1 mmol-1，吸光度测定过程处于线性期。求血清中LD含量

【参考答案】

根据酶活性U/L=ΔA/min×103×VT/（mε×l×V）有： V．T：反应液总体积，V：血清体积，l：比色皿光径，......

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